PMP22 Mouse Monoclonal Antibody [Clone ID: OTI5D1]

CAT#: CF808964

Carrier-free (BSA/glycerol-free) PMP22 mouse monoclonal antibody,clone OTI5D1

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PMP22 (RC216500, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PMP22. Positive lysates LY424991 (100ug) and LC424991 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PMP22 mouse monoclonal antibody.This figure shows negative staining. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808964) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PMP22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808964) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PMP22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808964) (1:2000)

Specifications

Product Data

• Clone Name: OTI5D1
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PMP22 (NP_000295) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 17.7 kDa
• Gene Name: Homo sapiens peripheral myelin protein 22 (PMP22), transcript variant 1, mRNA.
• Database Link:
  NP_000295
  Entrez Gene 24660 RatEntrez Gene 5376 Human
  UniProt ID Q01453
• Background: This gene encodes an integral membrane protein that is a major component of myelin in the peripheral nervous system. Studies suggest two alternately used promoters drive tissue-specific expression. Various mutations of this gene are causes of Charcot-Marie-Tooth disease Type IA, Dejerine-Sottas syndrome, and hereditary neuropathy with liability to pressure palsies. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
• Synonyms: CMT1A; CMT1E; DSS; GAS-3; GAS3; HMSNIA; HNPP; Sp110

Reference Data

• Protein Families: Transmembrane