Nestin (NES) Mouse Monoclonal Antibody [Clone ID: OTI3B12]

CAT#: CF809183

Carrier-free (BSA/glycerol-free) NES mouse monoclonal antibody,clone OTI3B12

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NES (RC218197, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NES (1:500).

• 

  Western blot analysis of extracts (35ug) from 3 different cell lines by using anti-NES monoclonal antibody (1:500).

• 

  Immunohistochemical staining of paraffin-embedded Human adult heart tissue within the normal limits using anti-NES mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809183) (1:2000)

• 

  Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum within the normal limits using anti-NES mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809183) (1:2000)

• 

  Immunohistochemical staining of paraffin-embedded Human placenta tissue within the normal limits using anti-NES mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809183) (1:2000)

• 

  Immunohistochemical staining of paraffin-embedded Human spleen tissue within the normal limits using anti-NES mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809183) (1:2000)

Show all 6 images

Specifications

Product Data

• Clone Name: OTI3B12
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 99-349 of human NES (NP_006608) produced in E.Coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens nestin (NES), mRNA.
• Database Link:
  NP_006608
  Entrez Gene 10763 Human
  UniProt ID P48681
• Synonyms: Nbla00170

Reference Data

• Protein Families: ES Cell Differentiation/IPS