Nestin (NES) Mouse Monoclonal Antibody [Clone ID: OTI3B12]

CAT#: TA809183S

NES mouse monoclonal antibody,clone OTI3B12

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NES (RC218197, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NES (1:500).

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  Western blot analysis of extracts (35ug) from 3 different cell lines by using anti-NES monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human adult heart tissue within the normal limits using anti-NES mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809183) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum within the normal limits using anti-NES mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809183) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human placenta tissue within the normal limits using anti-NES mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809183) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human spleen tissue within the normal limits using anti-NES mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809183) (1:2000)

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Specifications

Product Data

• Clone Name: OTI3B12
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 99-349 of human NES (NP_006608) produced in E.Coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: nestin
• Database Link:
  NP_006608
  Entrez Gene 10763 Human
  UniProt ID P48681
• Synonyms: Nbla00170

Reference Data

• Protein Families: ES Cell Differentiation/IPS