L3MBTL3 Mouse Monoclonal Antibody [Clone ID: OTI6D7]

CAT#: CF809704

Carrier-free (BSA/glycerol-free) L3MBTL3 mouse monoclonal antibody,clone OTI6D7

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY L3MBTL3 (RC209288, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-L3MBTL3 (1:2000). Positive lysates LY422800 (100ug) and LC422800 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-L3MBTL3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809704) (1:1000)

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-L3MBTL3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809704) (1:1000)

Specifications

Product Data

• Clone Name: OTI6D7
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:1000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 98-319 of human L3MBTL3 (NP_001007103) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 85.6 kDa
• Gene Name: Homo sapiens L3MBTL histone methyl-lysine binding protein 3 (L3MBTL3), transcript variant 2, mRNA.
• Database Link:
  NP_001007103
  Entrez Gene 237339 MouseEntrez Gene 309550 RatEntrez Gene 84456 Human
  UniProt ID Q96JM7
• Background: Putative Polycomb group (PcG) protein. PcG proteins maintain the transcriptionally repressive state of genes, probably via a modification of chromatin, rendering it heritably changed in its expressibility. Required for normal maturation of myeloid progenitor cells. [UniProtKB/Swiss-Prot Function]
• Synonyms: MBT-1; MBT1