TRIP13 Mouse Monoclonal Antibody [Clone ID: OTI2F5]

CAT#: CF809737

Carrier-free (BSA/glycerol-free) TRIP13 mouse monoclonal antibody,clone OTI2F5

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIP13 (RC228224, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIP13 (1:2000).

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  Western blot analysis of extracts (35ug) from 3 different cell lines by using anti-TRIP13 monoclonal antibody (293T: human; HeLa: human; Jurkat: human; ) (1:500).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and TRIP13-Knockout 293T cells (KO, Cat# LC811410) were separated by SDS-PAGE and immunoblotted with anti-TRIP13 monoclonal antibody TA809737, (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TRIP13 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809737) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-TRIP13 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809737) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TRIP13 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809737) (1:2000)

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Specifications

Product Data

• Clone Name: OTI2F5
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 31-148 of human TRIP13(NP_001159732) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens thyroid hormone receptor interactor 13 (TRIP13), transcript variant 2, mRNA.
• Database Link:
  NP_001159732
  Entrez Gene 69716 MouseEntrez Gene 292206 RatEntrez Gene 9319 Human
  UniProt ID Q15645
• Background: This gene encodes a protein that interacts with thyroid hormone receptors, also known as hormone-dependent transcription factors. The gene product interacts specifically with the ligand binding domain. This gene is one of several that may play a role in early-stage non-small cell lung cancer. [provided by RefSeq, Oct 2009]
• Synonyms: 16E1BP

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors