POLR2A Mouse Monoclonal Antibody [Clone ID: OTI1E5]

CAT#: CF809994

Carrier-free (BSA/glycerol-free) POLR2A mouse monoclonal antibody,clone OTI1E5

Formulation: Standard Carrier Free


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USD 465.00

In Stock*

Size
    • 100 ug

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Specifications

Product Data
Clone Name OTI1E5
Applications IHC, WB
Recommended Dilution WB 1:2000, IHC 1:500
Reactivities Human, Mouse, Rat
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Human recombinant protein fragment corresponding to amino acids 1351-1614 of human POLR2A(NP_000928) produced in E.coli.
Formulation Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Gene Name Homo sapiens RNA polymerase II subunit A (POLR2A), mRNA.
Background This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. [provided by RefSeq, Jul 2008]
Synonyms hRPB220; hsRPB1; POLR2; POLRA; RPB1; RPBh1; RpIILS; RPO2; RPOL2
Reference Data
Protein Pathways Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase

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