POLR2A Mouse Monoclonal Antibody [Clone ID: OTI3C8]

CAT#: CF810050

Carrier-free (BSA/glycerol-free) POLR2A mouse monoclonal antibody,clone OTI3C8

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLR2A (RC216765, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLR2A (1:500).

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  Western blot analysis of extracts (35ug) from 5 different cell lines by using anti-POLR2A monoclonal antibody. 1:500`

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-POLR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810050) (1:500)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-POLR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810050) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-POLR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810050) (1:500)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-POLR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810050) (1:500)

• Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-POLR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810050) (1:500)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-POLR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810050) (1:500)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-POLR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810050) (1:500)

• Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-POLR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810050) (1:500)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-POLR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810050) (1:500)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-POLR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810050) (1:500)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-POLR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810050) (1:500)

• Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-POLR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810050) (1:500)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-POLR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810050) (1:500)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-POLR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810050) (1:500)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-POLR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810050) (1:500)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-POLR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810050) (1:500)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-POLR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810050) (1:500)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-POLR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810050) (1:500)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-POLR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810050) (1:500)

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Specifications

Product Data

• Clone Name: OTI3C8
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1351-1614 of human POLR2A(NP_000928) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens RNA polymerase II subunit A (POLR2A), mRNA.
• Database Link:
  NP_000928
  Entrez Gene 20020 MouseEntrez Gene 363633 RatEntrez Gene 5430 Human
  UniProt ID P24928
• Synonyms: hRPB220; hsRPB1; POLR2; POLRA; RPB1; RPBh1; RpIILS; RPO2; RPOL2

Reference Data

• Protein Pathways: Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase