ZRANB1 Mouse Monoclonal Antibody [Clone ID: OTI7A3]

CAT#: CF810163

Carrier-free (BSA/glycerol-free) ZRANB1 mouse monoclonal antibody,clone OTI7A3

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZRANB1 (RC220808, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZRANB1 (1:2000). Positive lysates LY413719 (100ug) and LC413719 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-ZRANB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810163) (1:2000)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ZRANB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810163) (1:2000)

Specifications

Product Data

• Clone Name: OTI7A3
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 208-296 of human ZRANB1(NP_060050) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 80.8 kDa
• Gene Name: Homo sapiens zinc finger RANBP2-type containing 1 (ZRANB1), mRNA.
• Database Link:
  NP_060050
  Entrez Gene 360216 MouseEntrez Gene 100360606 RatEntrez Gene 54764 Human
  UniProt ID Q9UGI0
• Synonyms: TRABID

Reference Data

• Protein Families: Druggable Genome