LZTS1 Mouse Monoclonal Antibody [Clone ID: OTI3D12]

CAT#: CF810241

Carrier-free (BSA/glycerol-free) LZTS1 mouse monoclonal antibody,clone OTI3D12

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LZTS1 (RC210424, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LZTS1 (1:2000). Positive lysates LY412145 (100ug) and LC412145 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from Jurkat cell lines by using anti-LZTS1 monoclonal antibody (1:500).

Specifications

Product Data

• Clone Name: OTI3D12
• Applications: WB
• Recommended Dilution: WB 1:500~2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human LZTS1 (NP_066300) produced in HEK293T Cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 66.4 kDa
• Gene Name: Homo sapiens leucine zipper tumor suppressor 1 (LZTS1), transcript variant 1, mRNA.
• Database Link:
  NP_066300
  Entrez Gene 211134 MouseEntrez Gene 266711 RatEntrez Gene 11178 Human
  UniProt ID Q9Y250
• Background: This gene encodes a tumor suppressor protein that is ubiquitously expressed in normal tissues. In uveal melanomas, expression of this protein is silenced in rapidly metastasizing and metastatic tumor cells but has normal expression in slowly metastasizing or nonmetastasizing tumor cells. This protein may have a role in cell-cycle control by interacting with the Cdk1/cyclinB1 complex. This gene is located on chromosomal region 8p22. Loss of heterozygosity (LOH) in the 8p arm is a common characteristic of many types of cancer. [provided by RefSeq, Nov 2009]
• Synonyms: F37; FEZ1

Reference Data

• Protein Families: Druggable Genome