LHX2 Mouse Monoclonal Antibody [Clone ID: OTI2H2]

CAT#: CF810303

Carrier-free (BSA/glycerol-free) LHX2 mouse monoclonal antibody,clone OTI2H2

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LHX2 (RC210786, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LHX2 (1:2000). Positive lysates LY401508 (100ug) and LC401508 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI2H2
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG3
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 220-406 of human LHX2 (NP_004780) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens LIM homeobox 2 (LHX2), mRNA.
• Database Link:
  NP_004780
  Entrez Gene 16870 MouseEntrez Gene 296706 RatEntrez Gene 9355 Human
  UniProt ID P50458
• Background: This gene encodes a protein belonging to a large protein family, members of which carry the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein may function as a transcriptional regulator. The protein can recapitulate or rescue phenotypes in Drosophila caused by a related protein, suggesting conservation of function during evolution. [provided by RefSeq, Jul 2008]
• Synonyms: hLhx2; LH2

Reference Data

• Protein Families: Transcription Factors