TTF1 (NKX2-1) Mouse Monoclonal Antibody [Clone ID: OTI1C5]

CAT#: CF810448

Carrier-free (BSA/glycerol-free) NKX2-1 mouse monoclonal antibody,clone OTI1C5

Product Images

Western blot analysis of extracts (35ug) from cell lines by using anti-NKX2-1 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-NKX2-1 mouse monoclonal antibody.This figure shows negative staing. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810448) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-NKX2-1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810448) (1:500)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NKX2-1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810448) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-NKX2-1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810448) (1:500)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NKX2-1 mouse monoclonal antibody. This figure shows negative staining. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810448) (1:500)

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Specifications

Product Data

• Clone Name: OTI1C5
• Applications: IHC, WB
• Recommended Dilution: IHC 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human NKX2-1 ( NP_003308) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens NK2 homeobox 1 (NKX2-1), transcript variant 2, mRNA.
• Database Link:
  NP_003308
  Entrez Gene 21869 MouseEntrez Gene 25628 RatEntrez Gene 7080 Human
  UniProt ID P43699
• Background: This gene encodes a protein initially identified as a thyroid-specific transcription factor. The encoded protein binds to the thyroglobulin promoter and regulates the expression of thyroid-specific genes but has also been shown to regulate the expression of genes involved in morphogenesis. Mutations and deletions in this gene are associated with benign hereditary chorea, choreoathetosis, congenital hypothyroidism, and neonatal respiratory distress, and may be associated with thyroid cancer. Multiple transcript variants encoding different isoforms have been found for this gene. This gene shares the symbol/alias 'TTF1' with another gene, transcription termination factor 1, which plays a role in ribosomal gene transcription. [provided by RefSeq, Feb 2014]
• Synonyms: BCH; BHC; EBP; NK-2; NKX2.1; NKX2A; NMTC1; T; TEBP; TITF1; TTF-1; TTF1

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors