GCM1 Mouse Monoclonal Antibody [Clone ID: OTI4E2]

CAT#: CF810518

Carrier-free (BSA/glycerol-free) GCM1 mouse monoclonal antibody,clone OTI4E2

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GCM1 (RC220319, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GCM1 (1:2000). Positive lysates LY418526 (100ug) and LC418526 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI4E2
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human GCM1 (NP_003634) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens glial cells missing transcription factor 1 (GCM1), mRNA.
• Database Link:
  NP_003634
  Entrez Gene 8521 Human
  UniProt ID Q9NP62
• Background: This gene encodes a DNA-binding protein with a gcm-motif (glial cell missing motif). The encoded protein is a homolog of the Drosophila glial cells missing gene (gcm). This protein binds to the GCM-motif (A/G)CCCGCAT, a novel sequence among known targets of DNA-binding proteins. The N-terminal DNA-binding domain confers the unique DNA-binding activity of this protein. [provided by RefSeq, Jul 2008]
• Synonyms: GCMA; hGCMa

Reference Data

• Protein Families: Transcription Factors