VGLL1 Mouse Monoclonal Antibody [Clone ID: OTI7B8]

CAT#: CF810723

Carrier-free (BSA/glycerol-free) VGLL1 mouse monoclonal antibody,clone OTI7B8

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VGLL1 (RC200600, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VGLL1. Positive lysates LY414087 (100ug) and LC414087 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI7B8
• Applications: WB
• Recommended Dilution: WB 1:200
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human VGLL1 (NP_057351) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 28.5 kDa
• Gene Name: vestigial like family member 1
• Database Link:
  NP_057351
  Entrez Gene 51442 Human
  UniProt ID Q99990
• Background: The protein encoded by this gene binds proteins of the TEA domain family of transcription factors (TEFs) through the Vg (vestigial) homology region found in its N-terminus. It may thus function as a specific coactivator for the mammalian TEFs. [provided by RefSeq, Sep 2009]
• Synonyms: TDU; VGL1

Reference Data

• Protein Families: Transcription Factors