TCP1 delta (CCT4) Mouse Monoclonal Antibody [Clone ID: OTI5E5]

CAT#: CF810841

Carrier-free (BSA/glycerol-free) CCT4 mouse monoclonal antibody,clone OTI5E5

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCT4 (RC214480, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CCT4. Positive lysates LY401934 (100ug) and LC401934 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 cell lines by using anti-CCT4 monoclonal antibody (1:500).

Specifications

Product Data

• Clone Name: OTI5E5
• Applications: WB
• Recommended Dilution: WB 1:500~2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CCT4 (NP_006421) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 57.7 kDa
• Gene Name: Homo sapiens chaperonin containing TCP1 subunit 4 (CCT4), transcript variant 1, mRNA.
• Database Link:
  NP_006421
  Entrez Gene 29374 RatEntrez Gene 10575 Human
  UniProt ID P50991
• Background: The chaperonin containing TCP1 (MIM 186980) complex (CCT), also called the TCP1 ring complex, consists of 2 back-to-back rings, each containing 8 unique but homologous subunits, such as CCT4. CCT assists the folding of newly translated polypeptide substrates through multiple rounds of ATP-driven release and rebinding of partially folded intermediate forms. Substrates of CCT include the cytoskeletal proteins actin (see MIM 102560) and tubulin (see MIM 191130), as well as alpha-transducin (MIM 139330) (Won et al., 1998 [PubMed 9819444]). [supplied by OMIM, Mar 2008]
• Synonyms: CCT-DELTA; Cctd; SRB

Reference Data

• Protein Families: Druggable Genome