SLU7 Mouse Monoclonal Antibody [Clone ID: OTI5A7]

CAT#: CF810915

Carrier-free (BSA/glycerol-free) SLU7 mouse monoclonal antibody,clone OTI5A7

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SLU7 (RC202113, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SLU7 (1:500). Positive lysates LY416650 (100ug) and LC416650 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from HepG2 cell lysates by using anti-SLU7 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-SLU7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810915) (1:1000)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SLU7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810915) (1:1000)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-SLU7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810915) (1:1000)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-SLU7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810915) (1:1000)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SLU7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810915) (1:1000)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SLU7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810915) (1:1000)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-SLU7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810915) (1:1000)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SLU7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810915) (1:1000)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-SLU7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810915) (1:1000)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-SLU7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810915) (1:1000)

• Immunohistochemical staining of paraffin-embedded Human gastric tissue within the normal limits using anti-SLU7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810915) (1:1000)

• Immunohistochemical staining of paraffin-embedded Human spleen tissue within the normal limits using anti-SLU7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810915) (1:1000)

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Specifications

Product Data

• Clone Name: OTI5A7
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:1000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-300 of human SLU7 (NP_006416) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 68.2 kDa
• Gene Name: Homo sapiens SLU7 homolog, splicing factor (SLU7), transcript variant 2, mRNA.
• Database Link:
  NP_006416
  Entrez Gene 193116 MouseEntrez Gene 303057 RatEntrez Gene 10569 Human
  UniProt ID O95391
• Background: Pre-mRNA splicing occurs in two sequential transesterification steps. The protein encoded by this gene is a splicing factor that has been found to be essential during the second catalytic step in the pre-mRNA splicing process. It associates with the spliceosome and contains a zinc knuckle motif that is found in other splicing factors and is involved in protein-nucleic acid and protein-protein interactions. [provided by RefSeq, Jul 2008]
• Synonyms: 9G8; hSlu7

Reference Data

• Protein Pathways: Spliceosome