RNF14 Mouse Monoclonal Antibody [Clone ID: OTI3A4]

CAT#: CF810955

Carrier-free (BSA/glycerol-free) RNF14 mouse monoclonal antibody,clone OTI3A4

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNF14 (RC223362, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNF14. Positive lysates LY405271 (100ug) and LC405271 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI3A4
• Applications: WB
• Recommended Dilution: WB 1:200
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-220 of human RNF14 (NP_899647) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 53.7 kDa
• Gene Name: Homo sapiens ring finger protein 14 (RNF14), transcript variant 4, mRNA.
• Database Link:
  NP_899647
  Entrez Gene 56736 MouseEntrez Gene 619577 RatEntrez Gene 9604 Human
  UniProt ID Q9UBS8
• Background: The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of this gene has been demonstrated to inhibit the AR-mediated growth of prostate cancer. This protein also interacts with class III ubiquitin-conjugating enzymes (E2s) and may act as a ubiquitin-ligase (E3) in the ubiquitination of certain nuclear proteins. Six alternatively spliced transcript variants encoding two distinct isoforms have been reported. [provided by RefSeq, Jan 2011]
• Synonyms: ARA54; HFB30; HRIHFB2038; TRIAD2

Reference Data

• Protein Families: Druggable Genome, Transcription Factors