RNASE9 Mouse Monoclonal Antibody [Clone ID: OTI4F12]

CAT#: CF811245

Carrier-free (BSA/glycerol-free) RNASE9 mouse monoclonal antibody,clone OTI4F12

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNASE9 (RC225233, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNASE9 (1:500). Positive lysates LY426320 (100ug) and LC426320 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human testicle tissue within the normal limits using anti-RNASE9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811245) (1:150)

Specifications

Product Data

• Clone Name: OTI4F12
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human RNASE9 (NP_001103826) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens ribonuclease A family member 9 (inactive) (RNASE9), transcript variant 5, mRNA.
• Database Link:
  NP_001103826
  Entrez Gene 390443 Human
  UniProt ID P60153
• Synonyms: h461; HEL128; RAK1

Reference Data

• Protein Families: Secreted Protein