Pea3 (ETV4) Mouse Monoclonal Antibody [Clone ID: OTI1A10]

CAT#: CF811405

Carrier-free (BSA/glycerol-free) ETV4 mouse monoclonal antibody,clone OTI1A10

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ETV4 (RC202010, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ETV4 (1:2000). Positive lysates LY421534 (100ug) and LC421534 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI1A10
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-276 of human ETV4 (NP_001073143) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 53.8 kDa
• Gene Name: Homo sapiens ETS variant 4 (ETV4), transcript variant 2, mRNA.
• Database Link:
  NP_001073143
  Entrez Gene 18612 MouseEntrez Gene 360635 RatEntrez Gene 2118 Human
  UniProt ID P43268
• Synonyms: E1A-F; E1AF; PEA3; PEAS3

Reference Data

• Protein Families: Druggable Genome, Transcription Factors