KEL Mouse Monoclonal Antibody [Clone ID: OTI5E6]

CAT#: CF811547

Carrier-free (BSA/glycerol-free) KEL mouse monoclonal antibody,clone OTI5E6

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KEL (RC201127, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KEL. (1:2. Positive lysates LY400148 (100ug) and LC400148 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI5E6
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 166-420 of human KEL (NP_000411) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 82.6 kDa
• Gene Name: Homo sapiens Kell metallo-endopeptidase (Kell blood group) (KEL), mRNA.
• Database Link:
  NP_000411
  Entrez Gene 3792 Human
  UniProt ID P23276
• Background: This gene encodes a type II transmembrane glycoprotein that is the highly polymorphic Kell blood group antigen. The Kell glycoprotein links via a single disulfide bond to the XK membrane protein that carries the Kx antigen. The encoded protein contains sequence and structural similarity to members of the neprilysin (M13) family of zinc endopeptidases. [provided by RefSeq, Jul 2008]
• Synonyms: CD238; ECE3

Reference Data

• Protein Families: Druggable Genome, Protease, Transmembrane