SHARPIN Mouse Monoclonal Antibody [Clone ID: OTI1D11]

CAT#: CF811609

Carrier-free (BSA/glycerol-free) SHARPIN mouse monoclonal antibody,clone OTI1D11

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SHARPIN (RC222012, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SHARPIN (1:2000). Positive lysates LY410637 (100ug) and LC410637 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI1D11
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-326 of human SHARPIN (NP_112236) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens SHANK associated RH domain interactor (SHARPIN), transcript variant 1, mRNA.
• Database Link:
  NP_112236
  Entrez Gene 81858 Human
  UniProt ID Q9H0F6
• Synonyms: SIPL1

Reference Data

• Protein Families: Druggable Genome