RACGAP1 Mouse Monoclonal Antibody [Clone ID: OTI4C5]

CAT#: CF811640

Carrier-free (BSA/glycerol-free) RACGAP1 mouse monoclonal antibody,clone OTI4C5

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RACGAP1 (RC226045, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RACGAP1 (1:2000). Positive lysates LY426645 (100ug) and LC426645 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 2 different cell lines by using anti-RACGAP1 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human testicle tissue within the normal limits using anti-RACGAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811640) (1:150)

Specifications

Product Data

• Clone Name: OTI4C5
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 123-238 of human RACGAP1 (NP_001119575) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens Rac GTPase activating protein 1 (RACGAP1), transcript variant 2, mRNA.
• Database Link:
  NP_001119575
  Entrez Gene 26934 MouseEntrez Gene 315298 RatEntrez Gene 29127 Human
  UniProt ID Q9H0H5
• Background: This gene encodes a GTPase-activating protein (GAP) that is a compoment of the centralspindlin complex. This protein binds activated forms of Rho GTPases and stimulates GTP hydrolysis, which results in negative regulation of Rho-mediated signals. This protein plays a regulatory role in cytokinesis, cell growth, and differentiation. Alternatively spliced transcript variants have been found for this gene. There is a pseudogene for this gene on chromosome 12. [provided by RefSeq, Feb 2016]
• Synonyms: CYK4; HsCYK-4; ID-GAP; MgcRacGAP

Reference Data

• Protein Families: Druggable Genome