RNF12 (RLIM) Mouse Monoclonal Antibody [Clone ID: OTI2D7]

CAT#: CF811692

Carrier-free (BSA/glycerol-free) RLIM mouse monoclonal antibody,clone OTI2D7

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RLIM (RC203442, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RLIM (1:2000).

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  Western blot analysis of extracts (35ug) from 3 different cell lines by using anti-RLIM monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-RLIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811692) (1:500)

Specifications

Product Data

• Clone Name: OTI2D7
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human RLIM (NP_899196) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens ring finger protein, LIM domain interacting (RLIM), transcript variant 2, mRNA.
• Database Link:
  NP_899196
  Entrez Gene 51132 Human
  UniProt ID Q9NVW2
• Background: The protein encoded by this gene is a RING-H2 zinc finger protein. It has been shown to be an E3 ubiquitin protein ligase that targets LIM domain binding 1 (LDB1/CLIM), and causes proteasome-dependent degradation of LDB1. This protein and LDB1 are co-repressors of LHX1/LIM-1, a homeodomain transcription factor. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Feb 2009]
• Synonyms: NY-REN-43; RNF12

Reference Data

• Protein Families: Druggable Genome, Transcription Factors