TSC22 domain family, member 4 (TSC22D4) Mouse Monoclonal Antibody [Clone ID: OTI3G6]

CAT#: CF811733

Carrier-free (BSA/glycerol-free) TSC22D4 mouse monoclonal antibody,clone OTI3G6

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TSC22D4 (RC200322, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TSC2. Positive lysates LY410651 (100ug) and LC410651 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 2 cell lines by using anti-TSC22D4 monoclonal antibody (1:500).

Specifications

Product Data

• Clone Name: OTI3G6
• Applications: WB
• Recommended Dilution: WB 1:500~2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-395 of human TSC22D4 (NP_112197) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 40.8 kDa
• Gene Name: Homo sapiens TSC22 domain family member 4 (TSC22D4), transcript variant 1, mRNA.
• Database Link:
  NP_112197
  Entrez Gene 81628 Human
  UniProt ID Q9Y3Q8
• Background: TSC22D4 is a member of the TSC22 domain family of leucine zipper transcriptional regulators (see TSC22D3; MIM 300506) (Kester et al., 1999 [PubMed 10488076]; Fiorenza et al., 2001 [PubMed 11707329]). [supplied by OMIM, Mar 2008]
• Synonyms: THG-1; THG1; TILZ2

Reference Data

• Protein Families: Transcription Factors