Achaete scute homolog 3 (ASCL3) Mouse Monoclonal Antibody [Clone ID: OTI1C5]

CAT#: CF811864

Carrier-free (BSA/glycerol-free) ASCL3 mouse monoclonal antibody,clone OTI1C5

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ASCL3 (RC220042, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ASCL3. Positive lysates LY412408 (100ug) and LC412408 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI1C5
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human Ascl3 (NP_065697) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 20.7 kDa
• Gene Name: Homo sapiens achaete-scute family bHLH transcription factor 3 (ASCL3), mRNA.
• Database Link:
  NP_065697
  Entrez Gene 56676 Human
  UniProt ID Q9NQ33
• Background: Basic helix-loop-helix transcription factors, such as ASCL3, are essential for the determination of cell fate and the development and differentiation of numerous tissues (Jonsson et al., 2004 [PubMed 15475265]). [supplied by OMIM, Mar 2008]
• Synonyms: bHLHa42; HASH3; SGN1