ACSL5 Mouse Monoclonal Antibody [Clone ID: OTI1C6]

CAT#: CF811884

Carrier-free (BSA/glycerol-free) ACSL5 mouse monoclonal antibody,clone OTI1C6

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACSL5 (RC213380, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACSL5. Positive lysates LY404321 (100ug) and LC404321 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from mouse brain tissue lysate by using anti-ACSL5 monoclonal antibody (1:500).

Specifications

Product Data

• Clone Name: OTI1C6
• Applications: WB
• Recommended Dilution: WB 1:500~2000
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 104-620 of human ACSL5 (NP_976313) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 75.8 kDa
• Gene Name: Homo sapiens acyl-CoA synthetase long chain family member 5 (ACSL5), transcript variant 2, mRNA.
• Database Link:
  NP_976313
  Entrez Gene 433256 MouseEntrez Gene 51703 Human
  UniProt ID Q9ULC5
• Background: The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in uterus and spleen, and in trace amounts in normal brain, but has markedly increased levels in malignant gliomas. This gene functions in mediating fatty acid-induced glioma cell growth. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
• Synonyms: ACS2; ACS5; FACL5

Reference Data

• Protein Families: Transmembrane
• Protein Pathways: Adipocytokine signaling pathway, Fatty acid metabolism, Metabolic pathways, PPAR signaling pathway