ETS1 associated protein II (TDP2) Mouse Monoclonal Antibody [Clone ID: OTI3G10]

CAT#: CF811981

Carrier-free (BSA/glycerol-free) TDP2 mouse monoclonal antibody,clone OTI3G10

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TDP2 (RC202015, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TDP2. Positive lysates LY413867 (100ug) and LC413867 (20ug) can be purchased separately from OriGene.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and TDP2-Knockout HeLa cells (KO, Cat# LC833387) were separated by SDS-PAGE and immunoblotted with anti-TDP2 monoclonal antibody TA811981 (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

Specifications

Product Data

• Clone Name: OTI3G10
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TDP2 (NP_057698) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 40.7 kDa
• Gene Name: Homo sapiens tyrosyl-DNA phosphodiesterase 2 (TDP2), mRNA.
• Database Link:
  NP_057698
  Entrez Gene 51567 Human
  UniProt ID O95551
• Background: This gene encodes a member of a superfamily of divalent cation-dependent phosphodiesterases. The encoded protein associates with CD40, tumor necrosis factor (TNF) receptor-75 and TNF receptor associated factors (TRAFs), and inhibits nuclear factor-kappa-B activation. This protein has sequence and structural similarities with APE1 endonuclease, which is involved in both DNA repair and the activation of transcription factors. [provided by RefSeq, Jul 2008]
• Synonyms: AD022; dJ30M3.3; EAP2; EAPII; hTDP2; TTRAP

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors