ROD1 (PTBP3) Mouse Monoclonal Antibody [Clone ID: OTI5C9]

CAT#: CF812036

Carrier-free (BSA/glycerol-free) PTBP3 mouse monoclonal antibody,clone OTI5C9

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTBP3 (RC211812, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTBP3. Positive lysates LY417476 (100ug) and LC417476 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 3 cell lines by using anti-PTBP3 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PTBP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812036) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PTBP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812036) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PTBP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812036) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PTBP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812036) (1:2000)

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Specifications

Product Data

• Clone Name: OTI5C9
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 300-552 of human PTBP3 (NP_005147) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 59.5 kDa
• Gene Name: Homo sapiens polypyrimidine tract binding protein 3 (PTBP3), transcript variant 1, mRNA.
• Database Link:
  NP_005147
  Entrez Gene 83515 RatEntrez Gene 230257 MouseEntrez Gene 9991 Human
  UniProt ID O95758
• Background: The protein encoded by this gene binds RNA and is a regulator of cell differentiation. The encoded protein preferentially binds to poly(G) and poly(U) sequences in vitro. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
• Synonyms: ROD1

Reference Data

• Protein Families: Druggable Genome