GRPEL1 Mouse Monoclonal Antibody [Clone ID: OTI6G6]

CAT#: CF812313

Carrier-free (BSA/glycerol-free) GRPEL1 mouse monoclonal antibody,clone OTI6G6

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRPEL1 (RC206086, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRPEL. Positive lysates LY410847 (100ug) and LC410847 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI6G6
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 28-217 of human GRPEL1 (NP_079472) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 24.1 kDa
• Gene Name: Homo sapiens GrpE like 1, mitochondrial (GRPEL1), mRNA.
• Database Link:
  NP_079472
  Entrez Gene 80273 Human
  UniProt ID Q9HAV7
• Background: Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. Seems to control the nucleotide-dependent binding of mitochondrial HSP70 to substrate proteins. [UniProtKB/Swiss-Prot Function]
• Synonyms: GrpE; HMGE; mt-GrpE#1