UPP2 Mouse Monoclonal Antibody [Clone ID: OTI9G9]

CAT#: CF812319

Carrier-free (BSA/glycerol-free) UPP2 mouse monoclonal antibody,clone OTI9G9

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UPP2 (RC207260, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UPP2. Positive lysates LY406610 (100ug) and LC406610 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI9G9
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human UPP2 (NP_775491) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 35.3 kDa
• Gene Name: Homo sapiens uridine phosphorylase 2 (UPP2), transcript variant 1, mRNA.
• Database Link:
  NP_775491
  Entrez Gene 76654 MouseEntrez Gene 295620 RatEntrez Gene 151531 Human
  UniProt ID O95045
• Background: Catalyzes the reversible phosphorylytic cleavage of uridine and deoxyuridine to uracil and ribose- or deoxyribose-1-phosphate. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis. Shows substrate specificity and accept uridine, deoxyuridine, and thymidine as well as the two pyrimidine nucleoside analogs 5-fluorouridine and 5-fluoro-2(')-deoxyuridine as substrates. [UniProtKB/Swiss-Prot Function]
• Synonyms: UDRPASE2; UP2; UPASE2

Reference Data

• Protein Pathways: Drug metabolism - other enzymes, Metabolic pathways, Pyrimidine metabolism