GM CSF Receptor alpha (CSF2RA) Mouse Monoclonal Antibody [Clone ID: OTI2B2]

CAT#: CF812521

Carrier-free (BSA/glycerol-free) CSF2RA mouse monoclonal antibody,clone OTI2B2

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CSF2RA (RC201204, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CSF2RA (1:500).

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  Flow cytometric analysis of living 293T cells transfected with CSF2RA overexpression plasmid (RC201204), Red)/empty vector (PS100001, Blue) using anti-CSF2RA antibody (TA812521). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).

Specifications

Product Data

• Clone Name: OTI2B2
• Applications: FC, WB
• Recommended Dilution: WB 1:500, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 23-320 of human CSF2RA (NP_758448) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 44.1 kDa
• Gene Name: Homo sapiens colony stimulating factor 2 receptor alpha subunit (CSF2RA), transcript variant 2, mRNA.
• Database Link:
  NP_758448
  Entrez Gene 1438 Human
  UniProt ID P15509
• Background: The protein encoded by this gene is the alpha subunit of the heterodimeric receptor for colony stimulating factor 2, a cytokine which controls the production, differentiation, and function of granulocytes and macrophages. The encoded protein is a member of the cytokine family of receptors. This gene is found in the pseudoautosomal region (PAR) of the X and Y chromosomes. Multiple transcript variants encoding different isoforms have been found for this gene, with some of the isoforms being membrane-bound and others being soluble. [provided by RefSeq, Jul 2008]
• Synonyms: alphaGMR; CD116; CDw116; CSF2R; CSF2RAX; CSF2RAY; CSF2RX; CSF2RY; GM-CSF-R-alpha; GMCSFR; GMR; SMDP4

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Jak-STAT signaling pathway, Pathways in cancer