GNAL Mouse Monoclonal Antibody [Clone ID: OTI2F3]

CAT#: CF812962

Carrier-free (BSA/glycerol-free) GNAL mouse monoclonal antibody,clone OTI2F3

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GNAL (RC208226, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GNAL (1:500).

Specifications

Product Data

• Clone Name: OTI2F3
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-120 of human GNAL (NP_892023) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 52.3 kDa
• Gene Name: Homo sapiens G protein subunit alpha L (GNAL), transcript variant 1, mRNA.
• Database Link:
  NP_892023
  Entrez Gene 14680 MouseEntrez Gene 24611 RatEntrez Gene 2774 Human
  UniProt ID P38405
• Background: Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. G(olf) alpha mediates signal transduction within the olfactory neuroepithelium and the basal ganglia. May be involved in some aspect of visual transduction, and in mediating the effect of one or more hormones/neurotransmitters. [UniProtKB/Swiss-Prot Function]
• Synonyms: DYT25

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Olfactory transduction