Papillary renal cell carcinoma (PRCC) Mouse Monoclonal Antibody [Clone ID: OTI5G7]

CAT#: CF812965

Carrier-free (BSA/glycerol-free) PRCC mouse monoclonal antibody,clone OTI5G7

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRCC (RC203805, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRCC. Positive lysates LY416956 (100ug) and LC416956 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 8 different cell lines by using anti-PRCC monoclonal antibody (1:500).

Specifications

Product Data

• Clone Name: OTI5G7
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 135-491 of human PRCC (NP_005964) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 52.2 kDa
• Gene Name: Homo sapiens proline rich mitotic checkpoint control factor (PRCC), mRNA.
• Database Link:
  NP_005964
  Entrez Gene 94315 MouseEntrez Gene 310687 RatEntrez Gene 5546 Human
  UniProt ID Q92733
• Background: This gene encodes a protein that may play a role in pre-mRNA splicing. Chromosomal translocations (X;1)(p11;q21) that result in fusion of this gene to TFE3 (GeneID 7030) have been associated with papillary renal cell carcinoma. A PRCC-TFE3 fusion protein is expressed in affected carcinomas and is likely associated with altered gene transactivation. This fusion protein has also been associated with disruption of the cell cycle. [provided by RefSeq, Aug 2010]
• Synonyms: RCCP1; TPRC

Reference Data

• Protein Families: Druggable Genome