MICA Mouse Monoclonal Antibody [Clone ID: OTI2F5]

CAT#: CF813288

Carrier-free (BSA/glycerol-free) MICA mouse monoclonal antibody, clone OTI2F5

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MICA/MICB (RC204447/RC222315, Middle/Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MICA.(1:1000)

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  Western blot analysis of extracts (35ug) from 4 cell lines lysates by using anti-MICA monoclonal antibody. (1:500)

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  Flow cytometric analysis of living 293T cells transfected with MICA overexpression plasmid (RC204447), Red)/empty vector (PS100001, Blue) using anti-MICA antibody (TA813288). Cells incubated with a non-specific antibody (Green) were used as isotype control.(1:100)

Specifications

Product Data

• Clone Name: OTI2F5
• Applications: FC, WB
• Recommended Dilution: WB 1:500-1000, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MICA (NP_000238) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 42.9 kDa
• Gene Name: Homo sapiens MHC class I polypeptide-related sequence A (MICA), transcript variant 1*001, mRNA.
• Database Link:
  NP_000238
  Entrez Gene 100507436 Human
  UniProt ID Q29983
• Background: This gene encodes the highly polymorphic major histocompatability complex class I chain-related protein A. The protein product is expressed on the cell surface, although unlike canonical class I molecules it does not seem to associate with beta-2-microglobulin. It is a ligand for the NKG2-D type II integral membrane protein receptor. The protein functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells. Variations in this gene have been associated with susceptibility to psoriasis 1 and psoriatic arthritis, and the shedding of MICA-related antibodies and ligands is involved in the progression from monoclonal gammopathy of undetermined significance to multiple myeloma. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2014].
• Synonyms: MIC-A; PERB11.1