Nucleostemin (GNL3) Mouse Monoclonal Antibody [Clone ID: OTI4E1]

CAT#: CF813298

Carrier-free (BSA/glycerol-free) GNL3 mouse monoclonal antibody,clone OTI4E1

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GNL3 (RC200066, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GNL3 (1:500).

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  Western blot analysis of extracts (35ug) from 3 cell lines lysates by using anti-GNL3 monoclonal antibody (1:500).

Specifications

Product Data

• Clone Name: OTI4E1
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human GNL3 (NP_055181) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 61.8 kDa
• Gene Name: Homo sapiens G protein nucleolar 3 (GNL3), transcript variant 1, mRNA.
• Database Link:
  NP_055181
  Entrez Gene 26354 Human
  UniProt ID Q9BVP2
• Background: The protein encoded by this gene may interact with p53 and may be involved in tumorigenesis. The encoded protein also appears to be important for stem cell proliferation. This protein is found in both the nucleus and nucleolus. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]
• Synonyms: C77032; E2IG3; NNP47; NS

Reference Data

• Protein Families: ES Cell Differentiation/IPS, Stem cell - Pluripotency