EEA1 Mouse Monoclonal Antibody [Clone ID: OTI2G7]

CAT#: CF813373

Carrier-free (BSA/glycerol-free) EEA1 mouse monoclonal antibody, clone OTI2G7

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EEA1 (RC215396, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EEA1.(1:1000)

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  Western blot analysis of extracts (35ug) from 2 cell lines lysates by using anti-EEA1 monoclonal antibody. (1:500)

Specifications

Product Data

• Clone Name: OTI2G7
• Applications: WB
• Recommended Dilution: WB 1:500-1000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-400 of human EEA1 (NP_003557) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 162.3 kDa
• Gene Name: Homo sapiens early endosome antigen 1 (EEA1), mRNA.
• Database Link:
  NP_003557
  Entrez Gene 216238 MouseEntrez Gene 314764 RatEntrez Gene 8411 Human
  UniProt ID Q15075
• Background: Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking. [UniProtKB/Swiss-Prot Function]
• Synonyms: MST105; MSTP105; ZFYVE2

Reference Data

• Protein Pathways: Endocytosis