IL2 Receptor alpha (IL2RA) Mouse Monoclonal Antibody [Clone ID: OTI2A5]

CAT#: CF813473

Carrier-free (BSA/glycerol-free) IL2RA mouse monoclonal antibody, clone OTI2A5

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL2RA (RC215768, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL2RA.(1:1000)

• 

  Flow cytometric analysis of living 293T cells transfected with IL2RA overexpression plasmid (RC215768), Red)/empty vector (PS100001, Blue) using anti-IL2RA antibody (TA813473). Cells incubated with a non-specific antibody (Green) were used as isotype control. （1：100）

• 

  Flow cytometric analysis of living PBMCs treated with 10ug/ml PHA for 72h (Right)/untreated (Left) using anti-IL2RA antibody (TA813473).(1:100).

• 

  Flow cytometric analysis of living PBMCs treated with 10ug/ml PHA for 72h using anti-IL2RA antibody (TA813473)(Right). Cells incubated with a non-specific antibody (Left) were used as isotype control.(1:100).

Specifications

Product Data

• Clone Name: OTI2A5
• Applications: FC, WB
• Recommended Dilution: WB 1:1000, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human IL2RA (NP_000408) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 30.82 kDa
• Gene Name: Homo sapiens interleukin 2 receptor subunit alpha (IL2RA), transcript variant 1, mRNA.
• Database Link:
  NP_000408
  Entrez Gene 3559 Human
  UniProt ID P01589
• Background: The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009]
• Synonyms: CD25; IDDM10; IL2R; IMD41; p55; TCGFR

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Endocytosis, Hematopoietic cell lineage, Jak-STAT signaling pathway