IL3RA Mouse Monoclonal Antibody [Clone ID: OTI4A10]

CAT#: CF813664

Carrier-free (BSA/glycerol-free) IL3RA mouse monoclonal antibody, clone OTI4A10

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL3RA (RC206420, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL3RA.(1:1000)

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  Western blot analysis of extracts (35ug) from 2 cell lines lysates by using anti-IL3RA monoclonal antibody. (1:500)

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  Flow cytometric analysis of living 293T cells transfected with IL3RA overexpression plasmid (RC206420), Red)/empty vector (PS100001, Blue) using anti-IL3RA antibody (TA813664). Cells incubated with a non-specific antibody (Green) were used as isotype control.（1：100）

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  Flow cytometric analysis of living Jurkat cells, using anti-IL3RA antibody(TA813664, Red), compared to an isotype control (green), and a PBS control (blue).(1:100)

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  Flow cytometric analysis of living KG-1a cells, using anti-IL3RA antibody(TA813664, Red), compared to an isotype control (green), and a PBS control (blue).(1:100)

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Specifications

Product Data

• Clone Name: OTI4A10
• Applications: FC, WB
• Recommended Dilution: WB 1:500-1000, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human IL3RA (NP_002174) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 43.33 kDa
• Gene Name: Homo sapiens interleukin 3 receptor subunit alpha (IL3RA), transcript variant 1, mRNA.
• Database Link:
  NP_002174
  Entrez Gene 3563 Human
  UniProt ID P26951
• Background: This is a receptor for interleukin-3. [UniProtKB/Swiss-Prot Function]
• Synonyms: CD123; hIL-3Ra; IL3R; IL3RAY; IL3RX; IL3RY

Reference Data

• Protein Families: Transmembrane
• Protein Pathways: Apoptosis, Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Jak-STAT signaling pathway