Peroxiredoxin 6 (PRDX6) Mouse Monoclonal Antibody [Clone ID: OTI3A4]

CAT#: CF813656

Carrier-free (BSA/glycerol-free) PRDX6 mouse monoclonal antibody, clone OTI3A4

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRDX6 (RC207780, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRDX6.(1:1000)

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  Western blot analysis of extracts (35ug) from 2 cell lines lysates by using anti-PRDX6 monoclonal antibody. (1:500)

Specifications

Product Data

• Clone Name: OTI3A4
• Applications: WB
• Recommended Dilution: WB 1:500-1000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human Prdx6 (NP_004896) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 25 kDa
• Gene Name: Homo sapiens peroxiredoxin 6 (PRDX6), mRNA.
• Database Link:
  NP_004896
  Entrez Gene 11758 MouseEntrez Gene 94167 RatEntrez Gene 9588 Human
  UniProt ID P30041
• Background: The protein encoded by this gene is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. [provided by RefSeq, Jul 2008].
• Synonyms: 1-Cys; aiPLA2; AOP2; HEL-S-128m; NSGPx; p29; PRX

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Methane metabolism, Phenylalanine metabolism