Calponin (CNN1) Mouse Monoclonal Antibody [Clone ID: OTI3H2]

CAT#: CF813853

Carrier-free (BSA/glycerol-free) Calponin1 mouse monoclonal antibody, clone OTI3H2

Product Images

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Calponin1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, Cat# TA813853)

• 

  Immunohistochemical staining of paraffin-embedded Human Gastric Carcinoma using anti-Calponin1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, Cat# TA813853)

Specifications

Product Data

• Clone Name: OTI3H2
• Applications: IHC
• Recommended Dilution: IHC 1:30
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 67-257 of human Calponin1 (NP_001290) produced in E.coli.
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33.2 kDa
• Gene Name: Homo sapiens calponin 1 (CNN1), transcript variant 1, mRNA.
• Database Link:
  NP_001290
  Entrez Gene 12797 MouseEntrez Gene 65204 RatEntrez Gene 1264 Human
  UniProt ID P51911
• Synonyms: HEL-S-14; Sm-Calp; SMCC