Chromogranin A (CHGA) Mouse Monoclonal Antibody [Clone ID: OTI12E5]

CAT#: CF814051

Carrier-free (BSA/glycerol-free) CHGA mouse monoclonal antibody, clone OTI12E5

Product Images

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CHGA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, Cat# TA814051)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CHGA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, Cat# TA814051)

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  Immunohistochemical staining of paraffin-embedded Human appendix tissue within the normal limits using anti-CHGA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, Cat# TA814051)

Specifications

Product Data

• Clone Name: OTI12E5
• Applications: IHC
• Recommended Dilution: IHC 1:50
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 241-457 of human CHGA (NP_001266) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 50.7 kDa
• Gene Name: Homo sapiens chromogranin A (CHGA), transcript variant 1, mRNA.
• Database Link:
  NP_001266
  Entrez Gene 1113 Human
  UniProt ID P10645
• Background: The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Two other peptides, catestatin and chromofungin, have antimicrobial activity and antifungal activity, respectively. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]
• Synonyms: CGA

Reference Data

• Protein Families: Druggable Genome, Secreted Protein