CD21 (CR2) Mouse Monoclonal Antibody [Clone ID: OTI6C7]

CAT#: CF814212

Carrier-free (BSA/glycerol-free) CD21 mouse monoclonal antibody, clone OTI6C7

Product Images

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD21 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, Cat# TA814212)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD21 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, Cat# TA814212)

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  Immunohistochemical staining of paraffin-embedded Human spleen tissue within the normal limits using anti-CD21 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, Cat# TA814212)

Specifications

Product Data

• Clone Name: OTI6C7
• Applications: IHC
• Recommended Dilution: IHC 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 208-587 of human CD21 (NP_001868) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 113 kDa
• Gene Name: Homo sapiens complement C3d receptor 2 (CR2), transcript variant 2, mRNA.
• Database Link:
  NP_001868
  Entrez Gene 1380 Human
  UniProt ID P20023
• Background: This gene encodes a membrane protein, which functions as a receptor for Epstein-Barr virus (EBV) binding on B and T lymphocytes. Genetic variations in this gene are associated with susceptibility to systemic lupus erythematosus type 9 (SLEB9). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
• Synonyms: C3DR; CD21; CR; CVID7; SLEB9

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: B cell receptor signaling pathway, Complement and coagulation cascades, Hematopoietic cell lineage