LOC100736962 Rabbit Polyclonal Antibody

CAT#: R1079HRPS

LOC100736962 rabbit polyclonal antibody, HRP, Purified


USD 540.00

3 Days*

Size
    • 100 ug

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Specifications

Product Data
Applications ELISA, WB
Recommended Dilution Suitable for immunoblotting (Western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays.
Recommended Dilutions:
Western blot: 1/1000-1/5000
Immunohistochemistry.
ELISA: 1/14000.
This product has been assayed against 1.0 ug of esterase [porcine liver] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,500 of the reconstitution concentration is suggested.
Reactivities Porcine
Host Rabbit
Clonality Polyclonal
Immunogen Esterase from porcine liver
Specificity This antibody detects porcine esterase. Cross reactivity against esterase from other tissues and species may occur but have not been specifically determined.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-rabbit serum as well as purified and partially purified Esterase [porcine liver].
Formulation 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
Label: HRP
State: Purified
State: Lyophilized purified Ig fraction
Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
Preservative: 0.01% Gentamicin sulfate (Do NOT add Sodium azide!)
Label: Horseradish peroxidase
Reconstitution Method Restore with 0.1 ml of deionized water (or equivalent).
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Purification Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation HRP
Synonyms CES2, SES1, Liver carboxylesterase 1, HMSE, Egasyn, hBr1
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.