LOC100736962 Rabbit Polyclonal Antibody

CAT#: R1079HRPS

LOC100736962 rabbit polyclonal antibody, HRP, Purified

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Suitable for immunoblotting (Western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays.
  Recommended Dilutions:
  Western blot: 1/1000-1/5000
  Immunohistochemistry.
  ELISA: 1/14000.
  This product has been assayed against 1.0 ug of esterase [porcine liver] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,500 of the reconstitution concentration is suggested.
• Reactivities: Porcine
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Esterase from porcine liver
• Specificity: This antibody detects porcine esterase. Cross reactivity against esterase from other tissues and species may occur but have not been specifically determined.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-rabbit serum as well as purified and partially purified Esterase [porcine liver].
• Formulation: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
  Label: HRP
  State: Purified
  State: Lyophilized purified Ig fraction
  Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
  Preservative: 0.01% Gentamicin sulfate (Do NOT add Sodium azide!)
  Label: Horseradish peroxidase
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: HRP
• Database Link:
  Entrez Gene 397478 Pig
• Synonyms: CES2, SES1, Liver carboxylesterase 1, HMSE, Egasyn, hBr1