YAP1 Rabbit Monoclonal Antibody [Clone ID: EP1674Y]

CAT#: TA300956

Rabbit Monoclonal Antibody against YAP1 (Clone EP1674Y)

Product Images

Western blot - YAP1 antibody [EP1674Y]; Anti-YAP1 antibody [EP1674Y] at 1/50000 dilution + Hela cell lysate at 10 ug.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 65 kDa.Observed band size : 65 kDa.

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  Western blot - YAP1 antibody [EP1674Y];.developed using the ECL technique.Predicted band size : 65 kDa.Observed band size : 70 kDa.Exposure time : 1 minute

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  Immunohistochemistry (Paraffin-embedded sections) - YAP1 antibody [EP1674Y]; Immunohistochemical staining of YAP1 in paraffin embedded human breast carcinoma tissue using TA300956 at a 1/100 dilution.

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  Flow Cytometry - YAP1 antibody; Overlay histogram showing HeLa cells stained with TA300956 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Specifications

Product Data

• Clone Name: EP1674Y
• Applications: FC, IHC, WB
• Recommended Dilution: WB: 1:25000 - 1:50000; IP: 1:70; FC: 1:50; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:100 - 1:250
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide corresponding to residues near the C-terminus of human YAP65 was used as an immunogen.
• Formulation: PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• Purification: Tissue culture supernatant
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 65 kDa
• Gene Name: Yes associated protein 1
• Database Link:
  NP_006097
  Entrez Gene 22601 MouseEntrez Gene 363014 RatEntrez Gene 10413 Human
  UniProt ID P46937
• Background: The transcriptional coactivator Yes-associated protein (YAP) has been shown to interact with and to enhance p73-dependent apoptosis in response to DNA damage. It has been shown that YAP requires the promyelocytic leukemia gene (PML) and nuclear body localization to coactivate p73. YAP imparts selectivity to p73 by promoting the activation of a subset of p53 and/or p73 target promoters (1). Akt promotes YAP localization to the cytoplasm, resulting in loss from the nucleus where it functions as a coactivator of transcription factors including p73. p73-mediated induction of Bax expression following DNA damage requires YAP function and is attenuated by Akt phosphorylation of YAP. YAP overexpression increases, while YAP depletion decreases, p73-mediated apoptosis following DNA damage, in an Akt inhibitable manner (2)
• Synonyms: COB1; YAP; YAP2; YAP65; YKI
• Note: Does not react with Mouse, Rat

Reference Data

• Protein Families: Druggable Genome