NQO1 Mouse Monoclonal Antibody [Clone ID: A180]

CAT#: TA301418

Mouse Monoclonal Antibody against NQO1 (A180) - Neuronal Injury Marker

Product Images

Western Blot: NQO-1 Antibody (A180) TA301418 - Detection of NQO1 in A549 lysates using NB 200-209. Lane 1: 0.5 ug/ml. Lane 2: 2 ug/ml. Exposure: 1 second.

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  Immunocytochemistry/Immunofluorescence: NQO-1 Antibody (A180) TA301418 - NQO1 antibody was tested in U2OS cells with Dylight 488 (green). Nuclei and beta-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

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  Simple Western: NQO-1 Antibody (A180) TA301418 - Simple Western lane view shows a specific band for NQO-1 in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

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  Flow Cytometry: NQO-1 Antibody (A180) TA301418 - An intracellular stain was performed on U-87 cells with NQO-1 [A180] Antibody [TA301418C] (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.

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  Flow (Intracellular): NQO-1 Antibody (A180) TA301418 - An intracellular stain was performed on U87MG cells with NQO1 (A180) antibody NBP2-24917 (blue) along with a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).

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  Flow Cytometry: NQO-1 Antibody (A180) TA301418 - An intracellular stain was performed on U-87 cells with NQO-1 [A180] Antibody TA301418AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

• Flow Cytometry: NQO-1 Antibody (A180) TA301418 - Detection of NQO-1 in Human A549 Cell Line by Flow Cytometry. Human A549 cell line was stained with Mouse Anti- NQO-1 Monoclonal Antibody (Catalog # TA301418, filled histogram), or Mouse IgG1 isotype control (Catalog # MAB002, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.

• Immunohistochemistry-Paraffin: NQO-1 Antibody (A180) TA301418 - Analysis of NQO1 in human breast cancer using DAB with hematoxylin counterstain.

• Immunohistochemistry-Paraffin: NQO-1 Antibody (A180) TA301418 - Immunohistochemistry showing Anti-NQ01 on formalin fixed paraffin embedded human kidney tissue

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Specifications

Product Data

• Clone Name: A180
• Applications: IHC, WB
• Recommended Dilution: Flow (Intracellular), Flow Cytometry: 1-2 ug/mL, Immunohistochemistry: 1:100, Immunocytochemistry/ Immunofluorescence: 1:50, Immunoprecipitation: 1:1000, Western Blot: 1:1000, Immunohistochemistry-Paraffin: 1:100, Immunohistochemistry-Frozen: 1:100, Simple Western: 1:100
• Reactivities: Human, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length recombinant NQO1 (from human lung).
• Formulation: Tris-glycine and 150mM NaCl and Sodium Azide
• Purification: IgG purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: NAD(P)H quinone dehydrogenase 1
• Database Link:
  NP_000894
  Entrez Gene 24314 RatEntrez Gene 1728 Human
  UniProt ID P15559
• Background: NQO1 is a 2-electron reductase that detoxifies quinones derived from the oxidation of phenolic metabolites of benzene. Individuals homozygous for the T/T form of the 609C-T polymorphism have an increased risk of benzene hematotoxicity.
• Synonyms: DHQU; DIA4; DTD; NMOR1; NMORI; QR1

Reference Data

• Protein Families: Druggable Genome