ATF 4 (ATF4) Rabbit Polyclonal Antibody

CAT#: TA302232

Rabbit Polyclonal Antibody against ATF4 (S245)

Product Images

Western blot analysis of ATF4 (arrow) using rabbit anti-ATF4 Antibody (S245) (Cat. #TA302232). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ATF4 gene (Lane 2) (Origene Technologies).

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  Western blot analysis of ATF4 Antibody (S245) Pab (Cat. #TA302232) pre-incubated without (lane 1) and with (lane 2) blocking peptide in WiDr cell line lysate. ATF4 Antibody (pS245) (arrow) was detected using the purified Pab.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:1000
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This ATF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 220-252 amino acids from human ATF4.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 38459 Da
• Gene Name: activating transcription factor 4
• Database Link:
  NP_001666
  Entrez Gene 468 Human
  UniProt ID P18848
• Background: ATF4 is a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). ATF4 belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain.
• Synonyms: CREB-2; CREB2; TAXREB67; TXREB

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: GnRH signaling pathway, Long-term potentiation, MAPK signaling pathway, Neurotrophin signaling pathway, Prostate cancer