HSPC150 (UBE2T) Goat Polyclonal Antibody

CAT#: TA302510

Goat Polyclonal Antibody against HSPC150 / UBE2T

Product Images

TA302510 optimised QC. Primary incubation 1 hour at room temperature. Image A: K562 cell lysate at primary Ab concentration 0.1µg/ml, Image B: THP-1 cell lysate at primary Ab concentration 0.3µg/ml. (Loaded 35µg protein in RIPA buffer, per lane). Detected by chemiluminescence.

• 

  TA302510 optimised QC. Primary incubation 1 hour at room temperature. Images A, B, C, D: A431, HEK293, HeLa, Jurkat nuclear cell lysate at primary Ab concentration 0.3µg/ml, Image E: HepG2 nuclear cell lysate at primary Ab concentration 0.1µg/ml. (Loaded 35µg protein in RIPA buffer, per lane). Detected by chemiluminescence.

• 

  TA302510 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

• 

  TA302510 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

• 

  TA302510 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Show all 5 images

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:16000. WB: 0.1-0.3µg/ml.
• Reactivities: Human
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-QLVGIEKKFHPDV, from the C Terminus of the protein sequence according to NP_054895.1.
• Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: lot specific
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 26144 Da
• Gene Name: ubiquitin conjugating enzyme E2 T
• Database Link:
  NP_054895
  Entrez Gene 29089 Human
  UniProt ID Q9NPD8
• Background: The covalent conjugation of ubiquitin to proteins regulates diverse cellular pathways and proteins. Ubiquitin is transferred to a target protein through a concerted action of a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), such as UBE2T, and a ubiquitin ligase (E3) (Machida et al., 2006 [PubMed 16916645]). [supplied by OMIM]
• Synonyms: FANCT; HSPC150; PIG50

Reference Data

• Protein Families: Druggable Genome