Aurora A (AURKA) Goat Polyclonal Antibody

CAT#: TA302646

Goat Polyclonal Antibody against Aurora kinase A / STK15

Product Images

TA302646 (0.1ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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  HEK293 overexpressing Aurora kinase A (RC212018) with C-terminal tag (DYKDDDDK) and probed with anti-DYKDDDDK in the left panel and with TA302646 in the right panel (vector-only transfection in first and fourth lanes).

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:128,000. WB: 0.1-0.3µg/ml.
• Reactivities: Human
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-QNKESASKQS, from the C Terminus of the protein sequence according to NP_003591.2.
• Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: lot specific
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 45.6 kDa
• Gene Name: aurora kinase A
• Database Link:
  NP_940835
  Entrez Gene 6790 Human
  UniProt ID O14965
• Background: The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
• Synonyms: AIK; ARK1; AURA; BTAK; PPP1R47; STK6; STK7; STK15

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Stem cell - Pluripotency
• Protein Pathways: Oocyte meiosis