NQO1 Goat Polyclonal Antibody

CAT#: TA302678

Goat Polyclonal Antibody against NQO1

Product Images

TA302678 staining (1ug/ml) of Human Lung lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

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  TA302678 (1ug/ml) staining of Rat (A) and Pig (B) Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence

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  TA302678 (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

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  TA302678 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ELISA: 1:64000. WB: 0.03-0.1µg/ml.IHC:5µg/ml;IF:5µg/ml
• Reactivities: Human, Mouse, Dog
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-SIPTDNQIKARK, from the C Terminus of the protein sequence according to NP_000894.1; NP_001020604.1; NP_001020605.1.
• Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: lot specific
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 30.7 kDa
• Gene Name: NAD(P)H quinone dehydrogenase 1
• Database Link:
  NP_000894
  Entrez Gene 18104 MouseEntrez Gene 24314 RatEntrez Gene 610935 DogEntrez Gene 1728 Human
  UniProt ID P15559
• Background: This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]
• Synonyms: DHQU; DIA4; DTD; NMOR1; NMORI; QR1

Reference Data

• Protein Families: Druggable Genome