RACGAP1 Goat Polyclonal Antibody

CAT#: TA302684

Goat Polyclonal Antibody against RACGAP1 / MgcRacGAP

Product Images

TA302684 (1ug/ml) staining of A431 nuclear (A), Jurkat (B), Jurkat nuclear (C) and negative control Human Pancreas (D) lysate. (35ug protein in RIPA buffer) Detected by chemiluminescence.

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  TA302684 (4ug/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

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  TA302684 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).

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  TA302684 Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB: 0.2-1µg/ml.
• Reactivities: Human
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-GRQGNFFASPMLK, from the C Terminus of the protein sequence according to NP_037409.1.
• Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: lot specific
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 70.8 kDa
• Gene Name: Rac GTPase activating protein 1
• Database Link:
  NP_037409
  Entrez Gene 486549 DogEntrez Gene 29127 Human
  UniProt ID Q9H0H5
• Background: Rho GTPases control a variety of cellular processes. There are 3 subtypes of Rho GTPases in the Ras superfamily of small G proteins: RHO (see MIM 165370), RAC (see RAC1; MIM 602048), and CDC42 (MIM 116952). GTPase-activating proteins (GAPs) bind activated forms of Rho GTPases and stimulate GTP hydrolysis. Through this catalytic function, Rho GAPs negatively regulate Rho-mediated signals. GAPs may also serve as effector molecules and play a role in signaling downstream of Rho and other Ras-like GTPases. [supplied by OMIM]
• Synonyms: CYK4; HsCYK-4; ID-GAP; MgcRacGAP

Reference Data

• Protein Families: Druggable Genome