Neurofilament (NEFM) Rabbit Monoclonal Antibody [Clone ID: EPR580(2)Y]

CAT#: TA303832

Rabbit monoclonal antibody against Neurofilament M Phospho (pS614/619) (EPR580(2)Y ) (phospho-specific)

Product Images

Western blot - Anti-160 kD Neurofilament Medium (phospho S614 + S619) antibody [EPR580 (2)Y]; All lanes : Anti-160 kD Neurofilament Medium (phospho S614 + S619) antibody [EPR580 (2)Y] at 1/500 dilution.Lane 1 : SH-SY5Y cell lysate treated with Alkaline phosphatase.Lane 2 : Untreated SH-SY5Y cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP conjugated Goat anti-rabbit at 1/2000 dilution.Predicted band size : 102 kDa.Observed band size : 160 kDa.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 160 kD Neurofilament Medium (phospho S614 + S619) antibody [EPR580 (2)Y]; Immunohistochemical analysis of paraffin-embedded human brain tissue using TA303832 at a dilution of 1/100 - 1/250

Specifications

Product Data

• Clone Name: EPR580(2)Y
• Applications: IHC, WB
• Recommended Dilution: WB: 1:500; IHC: 1:100 - 250; IP: 1:30
• Reactivities: Human
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Serine 614/619 of human NF-M was used as an immunogen.
• Formulation: pH: 7.40
  Preservative: 0.01% Sodium azide
  Constituents: 50% Glycerol, 0.05% BSA
• Purification: Tissue culture supernatant
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 102 kDa
• Gene Name: neurofilament, medium polypeptide
• Database Link:
  NP_005373
  Entrez Gene 4741 Human
  UniProt ID P07197
• Background: Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq]
• Synonyms: NEF3; NF-M; NFM

Reference Data

• Protein Pathways: Amyotrophic lateral sclerosis (ALS)